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Product Name
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CD5 antibody [B19.1] (Allophycocyanin)
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See all CD5 antibodies (52)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [B19.1] to CD5 (Allophycocyanin)
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Immunogen
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The details of the immunogen for this antibody are not available.
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Reacts with
(species key)
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Ms
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Specificity
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ab25279 recognises CD5/Lyt-1 antigen.
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Tested applications
(see key)
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CellAct, Flow Cyt, IHC-Fr, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Cellular activation (activation of T cells in vitro): Use at an assay dependent dilution.
Flow Cyt: Use 0.2µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Type I membrane protein
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD
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Relevance
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In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 12507 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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B19.1
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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IgG fraction
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Storage buffer
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Preservative: Sodium Azide
Constituents: PBS and a stabilizing agent Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Conjugation
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Allophycocyanin
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C in the dark. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD5 antibody [B19.1] (Allophycocyanin) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CD5
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CD5 antibody [B19.1] (Allophycocyanin) - more information
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