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Products:Immunology >> Adaptive Immunity >> B Cells >> CD
Overview
Product name
Anti-CD5 antibody [B19.1] (Allophycocyanin)
See all CD5 products (60) ...
Description
Rat monoclonal [B19.1] to CD5 (Allophycocyanin)
Conjugation
Allophycocyanin
Specificity
ab25279 recognises CD5/Lyt-1 antigen.
Tested applications
Cellular activation, WB, Flow Cyt, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Mouse
Immunogen
The details of the immunogen for this antibody are not available.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS: Stabilizing agent
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
B19.1
Isotype
IgG2a
Light chain type
kappa
Research areas
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> B Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Immunology >> Adaptive Immunity >> B Cells >> CD
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cellular activation
WB
Flow Cyt
IHC-Fr
IP
Application notes
Cellular activation (activation of T cells in vitro): Use at an assay dependent dilution.
Flow Cyt: Use 0.2µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
Sequence similarities
Contains 3 SRCR domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP06127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 5 antibody
- CD5 antibody
- CD5 antibody
see all
Database links
- Entrez Gene: 12507 Mouse
- SwissProt: P13379 Mouse
- Unigene: 779 Mouse
References for Anti-CD5 antibody [B19.1] (Allophycocyanin) (ab25279)
ab25279 has not yet been referenced specifically in any publications.
Publishing research using ab25279? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"