Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins
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ab7166 has been referenced in 6 publications.
Publishing research using ab7166? Please let us know so that we can cite the reference in this datasheet
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ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Sharon Sneddon
ab7166 staining CD51 and CD61 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody.
This image is a courtesy of Olivia Merkel
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