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Immunocytochemistry/ Immunofluorescence - CD51 + CD61 antibody [BV3] (ab7166)
![Immunocytochemistry/ Immunofluorescence - CD51 + CD61 antibody [BV3] (ab7166)](/ps/datasheet/images/ab7166_review_12349.jpg)
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Flow Cytometry - CD51 + CD61 antibody [BV3] (ab7166)
![Flow Cytometry - CD51 + CD61 antibody [BV3] (ab7166)](/ps/datasheet/images/7/ab7166/CD51-CD61-Primary-antibodies-ab7166-4.jpg)
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Product Name
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CD51 + CD61 antibody [BV3]
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See all CD51 + CD61 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [BV3] to CD51 + CD61
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Reacts with
(species key)
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Hu, Rat, Chk
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Specificity
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This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: 1/250.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not suitable for use in Western Blot.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; single-pass type I membrane protein.
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Mast Cell Lineage
Immunology >> Cell Type Markers >> CD >> Adhesion
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Beta
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Integrins >> Alpha
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Integrins
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Relevance
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Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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BV3
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.2% BSA, 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD51 + CD61 antibody [BV3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD51 + CD61 antibody [BV3] images, references, products related to ab7166 and other tools.
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CD51 + CD61 antibody [BV3] images:
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 Immunocytochemistry/ Immunofluorescence - CD51 + CD61 antibody [BV3] (ab7166)
ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary. This image is courtesy of an Abreview submitted by Dr Sharon Sneddon
See Abreview
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Flow Cytometry - CD51 + CD61 antibody [BV3] (ab7166)
ab7166 staining CD51 and CD61 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody. This image is a courtesy of Olivia Merkel
See Abreview
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References for CD51 + CD61 antibody [BV3] (ab7166)
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This product has been used in: (two most recent references)
Merkel OM et al. Integrin alphaVbeta3 targeted gene delivery using RGD peptidomimetic conjugates with copolymers of PEGylated poly(ethylene imine). Bioconjug Chem 20:1270-80 (2009). PubMed: 19476331
Dare EV et al. Fibrin sealants from fresh or fresh/frozen plasma as scaffolds for in vitro articular cartilage regeneration. Tissue Eng Part A 15:2285-97 (2009). PubMed: 19226200
See all 3 publication references for this product.
If you publish research using ab7166 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD51 + CD61
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CD51 + CD61 antibody [BV3] - more information
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Customer reviews (feedback) regarding CD51 + CD61 antibody [BV3] |
Customer FAQs regarding CD51 + CD61 antibody [BV3] |
Protocols for CD51 + CD61 antibody [BV3] |
Price and availability of products related to CD51 + CD61 antibody [BV3] |
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CD51 + CD61 antibody [BV3] - related products:
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CD51 + CD61 antibody [BV3] - other tools:
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Contact Abcam with a Technical Enquiry about CD51 + CD61 antibody [BV3] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - CD51 + CD61 antibody [BV3] (ab7166)](/index.html?datasheet=7166#ab7166_review_12349.jpg)
![Flow Cytometry - CD51 + CD61 antibody [BV3] (ab7166)](/index.html?datasheet=7166#CD51-CD61-Primary-antibodies-ab7166-4.jpg)
