CD53 antibody [MEM-53] (FITC) (ab68565)

Product Name 

CD53 antibody [MEM-53] (FITC)

Product type 

Primary antibodies

Description 

Mouse monoclonal [MEM-53] to CD53 (FITC)

Immunogen 

Leukocytes of patient suffering from LGL-type leukemia.

Reacts with 
(species key)

Hu

Specificity 

ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).

Tested applications 
(see key)

Flow Cyt

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use Neat: 20µl per 100µl of whole blood or 10⁶ cells.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human blood cells.

Cellular localization 

Membrane; Multi-pass membrane protein.

Research areas 

Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage

Relevance 

CD53 is a member of the tetraspan family of molecules, and is expressed by all leucocytes, but is absent from red cells and platelets.
Most of the tetraspan family of molecules are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2 generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MEM-53

Isotype 

IgG1

Purity 

Size exclusion

Conjugation notes 

ab68565 is conjugated with Fluorescein isothiocyanate (FITC) under optimum
conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Storage instructions 

Store at +4°C in the dark. Do not freeze.

References for CD53 antibody [MEM-53] (FITC) (ab68565)

  This clone has been used in: (two most recent references)

Rasmussen AM et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994). PubMed: 7963560

Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
If you publish research using ab68565 please let us know so that we can cite the reference on this datasheet.

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