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Anti-CD53 antibody [MEM-53] (FITC)
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Mouse monoclonal [MEM-53] to CD53 (FITC)
FITC
ab68565 is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Flow Cytmore details
Reacts with
Human
Leukocytes of patient suffering from LGL-type leukemia.
Human blood cells.
Liquid
Store at +4°C in the dark. Do not freeze.
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
Size exclusion
Monoclonal
MEM-53
IgG1
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage
Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)
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Our Abpromise guarantee covers the use of ab68565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells. (Use neat)
May be involved in growth regulation in hematopoietic cells.
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Belongs to the tetraspanin (TM4SF) family.
Membrane.
Target information above from: UniProt accessionP19397
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)
![Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)](/ps/datasheet/images/68/ab68565/CD53-Primary-antibodies-ab68565-1.jpg)
Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
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![Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)](/ps/datasheet/images/68/ab68565/CD53-Primary-antibodies-ab68565-1.jpg)
Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
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