![Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565) image](#CD53-Primary-antibodies-ab68565-1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD53 antibody [MEM-53] (FITC)
See all CD53 products (9) ...
Description
Mouse monoclonal [MEM-53] to CD53 (FITC)
Conjugation
FITC
Conjugation notes
ab68565 is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Specificity
ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Tested applications
Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Leukocytes of patient suffering from LGL-type leukemia.
Positive control
Human blood cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration
Concentration information loading...
Purity
Size exclusion
Clonality
Monoclonal
Clone number
MEM-53
Isotype
IgG1
Research areas
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)
![Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565) image](/ps/datasheet/images/68/ab68565/CD53-Primary-antibodies-ab68565-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab68565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 106 cells. (Use neat)
Target
Function
May be involved in growth regulation in hematopoietic cells.
Tissue specificity
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP19397
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Tetraspanin 25 antibody
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC OX44 antibody
see all
Database links
- Entrez Gene: 963 Human
- Omim: 151525 Human
- SwissProt: P19397 Human
- Unigene: 443057 Human
Anti-CD53 antibody [MEM-53] (FITC) images:
Flow Cytometry-Anti-CD53 antibody [MEM-53] (FITC)(ab68565)
Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
References for Anti-CD53 antibody [MEM-53] (FITC) (ab68565)
This product has been referenced in:
- Rasmussen AMet al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994).Read more (PubMed: 7963560) »
- Bazil Vet al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989).Read more (PubMed: 2534480) »
See all 2 publications for this product
Publishing research using ab68565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"