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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD53 antibody [MEM-53]
See all CD53 products (9) ...
Description
Mouse monoclonal [MEM-53] to CD53
Specificity
Human CD53 antigen. This antibody also recognizes the deglycosylated antigen (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Tested applications
Flow Cyt, IHC-Fr, IP, WB, ICC/IF, Functional Studiesmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human). (Leucocytes from a patient suffering from a LGL-type leukaemia).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by protein A-affinity chromatography.
Primary antibody notes
The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.
Clonality
Monoclonal
Clone number
MEM-53
Isotype
IgG1
Research areas
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage
Applications
Show applications key
Our Abpromise guarantee covers the use of ab667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 4 µg/ml
IHC-Fr: Use at an assay dependent concentration. (Can be used to discriminate lymphomas from other tumors.)
IP: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution. (PubMed: 17438075)
FuncS: Use at an assay dependent dilution. (Induces activation of monocytes and B lymphocytes.)
Target
Function
May be involved in growth regulation in hematopoietic cells.
Tissue specificity
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP19397
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
Database links
- Entrez Gene: 963 Human
- Omim: 151525 Human
- SwissProt: P19397 Human
- Unigene: 443057 Human
References for Anti-CD53 antibody [MEM-53] (ab667)
This product has been referenced in:
- Ruiz-Mateos Eet al. CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages. J Virol 82:4751-61 (2008). ICC/IF.Read more (PubMed: 18321974) »
- Deneka Met al. In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. J Cell Biol 177:329-41 (2007). ICC/IF.Read more (PubMed: 17438075) »
See all 7 publications for this product
Publishing research using ab667? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"