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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD53 antibody [MRC OX-44]
See all CD53 products (9) ...
Description
Mouse monoclonal [MRC OX-44] to CD53
Tested applications
Flow Cyt, IHC-Fr, IHC-P, IPmore details
Cross reactivity
Reacts with
Rat
Immunogen
Tissue / cell preparation (Rat).Rat T cell blasts
Epitope
Clone OX-44 recognises a conformational epitope that is dependent on disulphide bonding.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Primary antibody notes
The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain.
Clonality
Monoclonal
Clone number
MRC OX-44
Myeloma
NS0
Isotype
IgG1
Research areas
Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
IHC-Fr
IHC-P
IP
Application notes
Flow Cyt: 1/100 - 1/200. Use 10ul of the suggested working dilution to label 106 cells in 100ul.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
(Note: ab22383 requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase)
IP: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in growth regulation in hematopoietic cells.
Tissue specificity
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Cellular localization
Membrane.
Target information above from: UniProt accessionP19397
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
Database links
References for Anti-CD53 antibody [MRC OX-44] (ab22383)
This product has been referenced in:
- Tomlinson MGet al. Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. Eur J Immunol 23:136-40 (1993).Read more (PubMed: 7678222) »
- Paterson DJet al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med 165:1-13 (1987).Read more (PubMed: 3098892) »
See all 2 publications for this product
Publishing research using ab22383? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"