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Anti-CD53 antibody [OX-80] (ab22484)

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Overview

Product name

Anti-CD53 antibody [OX-80]
See all CD53 products (9) ...

Description

Rat monoclonal [OX-80] to CD53

Specificity

ab22484 recognises CD53 cell surface antigen. It recognises an extracellular epitope and its binding is dependent upon the threonine residue at position 154.

Tested applications

Flow Cytmore details

Cross reactivity

Reacts with

Mouse

Immunogen

Tissue / cell preparation (Mouse): RAW264 cell line

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Tissue culture supernatant

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

OX-80

Isotype

IgM

Research areas

Signal Transduction >> Adapters >> Transmembrane
Immunology >> Cell Type Markers >> CD >> Non-lineage

Applications

Show applications key

Our Abpromise guarantee covers the use of ab22484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     

Application notes

Flow Cyt: Use neat - 1/5. Use 10µl of the suggested working dilution for 106 cells.


Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be involved in growth regulation in hematopoietic cells.

Tissue specificity

B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Cellular localization

Membrane.

Target information above from: UniProt accessionP19397 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AI323659 antibody
  • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
  • CD 53 antibody
  • CD53 antibody
  • CD53 antigen antibody
  • CD53 antigen, isoform CRA_b antibody
  • Cd53 molecule antibody
  • CD53_HUMAN antibody
  • Cell surface glycoprotein CD53 antibody
  • Leukocyte antigen MRC OX-44 antibody
  • Leukocyte surface antigen CD53 antibody
  • MOX 44 antibody
  • MOX44 antibody
  • OTTHUMP00000059505 antibody
  • Ox-44 antibody
  • OX44 antibody
  • RATOX44 antibody
  • Tetraspanin 25 antibody
  • Tetraspanin 25 antibody
  • Tetraspanin-25 antibody
  • Tetraspanin25 antibody
  • Transmembrane glycoprotein antibody
  • TSPAN 25 antibody
  • Tspan-25 antibody
  • TSPAN25 antibody
see all

Database links

References for Anti-CD53 antibody [OX-80] (ab22484)

ab22484 has not yet been referenced specifically in any publications.

Publishing research using ab22484? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"