CD55 293T Transfected Lysate - (positive control) (ab94035)

Overview

  • Product name
    CD55 293T Transfected Lysate - (positive control)
  • General notes
    ab94035 is a 293T cell transfected lysate in which Human CD55 has been transiently over-expressed using a pCMV-CD55 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Tissue specificity: Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. Similarity: Belongs to the receptors of complement activation (RCA) family. Contains 4 Sushi (CCP/SCR) domains. Domain: The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. PTM: The Ser/Thr-rich domain is heavily O-glycosylated.

Applications

Our Abpromise guarantee covers the use of ab94035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94035 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-CD55 antibody (ab54595) at 1/500 dilution

    Lane 1 : CD55 293T Transfected Lysate - (positive control) (ab94035)
    Lane 2 : 293T non-Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94035 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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