![Flow Cytometry-Anti-CD55 antibody [143-30] (FITC)(ab25634) image](#CD55-Primary-antibodies-ab25634-1.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD55 antibody [143-30] (FITC)
See all CD55 products (17) ...
Description
Mouse monoclonal [143-30] to CD55 (FITC)
Conjugation
FITC
Specificity
ab25634 recognises CD55, also known as decay accelerating factor (DAF).
Tested applications
Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Human, Pig
Immunogen
The details of the immunogen for this antibody are not available.
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
143-30
Isotype
IgG1
Research areas
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Flow Cytometry-Anti-CD55 antibody [143-30] (FITC)(ab25634)
![Flow Cytometry-Anti-CD55 antibody [143-30] (FITC)(ab25634) image](/ps/datasheet/images/25/ab25634/CD55-Primary-antibodies-ab25634-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab25634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 5µl for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains.
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
Post-translational
modifications
The Ser/Thr-rich domain is heavily O-glycosylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Decay accelerating factor (GPI-form) antibody
- CD 55 antibody
- CD55 antibody
see all
Database links
- Entrez Gene: 1604 Human
- Entrez Gene: 396743 Pig
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
Anti-CD55 antibody [143-30] (FITC) images:
Flow Cytometry-Anti-CD55 antibody [143-30] (FITC)(ab25634)
Overlay histogram showing peripheral blood lymphocytes stained with ab25634 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25634, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
References for Anti-CD55 antibody [143-30] (FITC) (ab25634)
This product has been referenced in:
- Hofmann Aet al. Proteomic cell surface phenotyping of differentiating acute myeloid leukemia cells. Blood 116:e26-34 (2010). Flow Cyt; Human.Read more (PubMed: 20570859) »
See 1 publication for this product
Publishing research using ab25634? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"