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Anti-CD55 antibody [MEM-118] (ab1422)

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1 question for ab1422

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Question 1

Friday 09-March-2012

What is the protocol that was used to test this antibody in IHC-P

ANSWER:

  

Thank you for contacting Abcam yesterday.

I asked the supplying lab for the IHC-P protocol they used to verify that the antibody works in IHC but they we do not have the protocol, only information that the suitable working concentration for IHC-paraffin sections by ab1422 is 10 ug/ml.

If you can send me a copy of the protocol that you are using, including the fix, then I will be happy to see if there are any suggestions that I can make. Also, does the secondary antibody you are using with the ab1422, work well with other primary antibodies and also are you able to stain the same sections successfully with other primary antibodies. I ask as I talked to one of my colleagues and they mentioned that they have seen this problem before and they found that it was due to the fixation process. if the sections are working well with other antibodies then the fixation is most likely not the cause but I wanted to check.

I look forward to you reply and helping you resolve this issue.

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