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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD55 antibody [MEM-118]
See all CD55 products (17) ...
Description
Mouse monoclonal [MEM-118] to CD55
Tested applications
IHC-P, Flow Cyt, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Non Human Primates
Immunogen
Tissue / cell preparation (Human). (T cell line HPB-ALL).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites by gel filtration and precipitation methods.
Clonality
Monoclonal
Clone number
MEM-118
Isotype
IgM
Research areas
Immunology >> Cell Type Markers >> CD >> Non-lineage
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/ml
Flow Cyt: Use a concentration of 10 µg/ml
IP: Use at an assay dependent concentration.
Target
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains.
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
Post-translational
modifications
The Ser/Thr-rich domain is heavily O-glycosylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Decay accelerating factor (GPI-form) antibody
- CD 55 antibody
- CD55 antibody
see all
Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
References for Anti-CD55 antibody [MEM-118] (ab1422)
This product has been referenced in:
- Schlossman (Eds.) et al. Leucocyte Typing V. Oxford University Press : (1995).Read more (PubMed: ) »
- Cinek T & Horejsi V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992).Read more (PubMed: 1382093) »
See all 2 publications for this product
Publishing research using ab1422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"