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Anti-CD55 antibody (ab37720) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP. at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
A fusion protein has been used in Western Blot hence the difference in predicted and actual MW.
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