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Western blot - CD55 antibody (ab37720)
(enlarge)
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Product Name
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CD55 antibody
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See all CD55 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to CD55
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Immunogen
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Synthetic peptide: CGLPPDVPNA QPALEGRTSF PEDTVITYKC EESFVKIPGE KDSVICLKGS QWSDIEEFCN QKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTG, corresponding to amino acids 36/129 of Human CD55
BLAST 'CGLPPDVPNA QPALEGRTSF PEDTVITYKC EESFVKIPGE KDSVICLKGS QWSDIEEFCN QKSCPNPGEI RNGQIDVPGG ILFGATISFS ' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for CD55.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/2000. Predicted molecular weight: 41 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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E. coli-derived fusion protein.
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Cellular localization
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Isoform 1: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Cell membrane; Lipid-anchor, I-anchor.
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Research areas
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Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins.
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Isotype
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IgY
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD55 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD55 antibody images, references, products related to ab37720 and other tools.
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CD55 antibody images:
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 Western blot - CD55 antibody (ab37720)
CD55 antibody (ab37720) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP. at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
A fusion protein has been used in Western Blot hence the difference in predicted and actual MW.
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Search PubMed (MEDLINE) for references to CD55
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CD55 antibody - more information
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Customer reviews (feedback) regarding CD55 antibody |
Customer FAQs regarding CD55 antibody |
Protocols for CD55 antibody |
Price and availability of products related to CD55 antibody |
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CD55 antibody - related products:
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CD55 antibody - other tools:
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Contact Abcam with a Technical Enquiry about CD55 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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