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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD55 antibody
See all CD55 products (17) ...
Description
Chicken polyclonal to CD55
Specificity
This antibody is specific for CD55.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: CGLPPDVPNA QPALEGRTSF PEDTVITYKC EESFVKIPGE KDSVICLKGS QWSDIEEFCN QKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTG, corresponding to amino acids 36/129 of Human CD55
CGLPPDVPNA QPALEGRTSF PEDTVITYKC EESFVKIPGE KDSVICLKGS QWSDIEEFCN QKSCPNPGEI RNGQIDVPGG ILFGATISFS CNTG
Positive control
E. coli-derived fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Western blot - CD55 antibody (ab37720)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Predicted molecular weight: 41 kDa.
Target
Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains.
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
Post-translational
modifications
The Ser/Thr-rich domain is heavily O-glycosylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP08174
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Decay accelerating factor (GPI-form) antibody
- CD 55 antibody
- CD55 antibody
see all
Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
Anti-CD55 antibody images:
Western blot - CD55 antibody (ab37720)
Anti-CD55 antibody (ab37720) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg
Secondary
Goat anti-IgY-HRP. at 1/1000 dilution
Predicted band size : 41 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
A fusion protein has been used in Western Blot hence the difference in predicted and actual MW.
References for Anti-CD55 antibody (ab37720)
ab37720 has not yet been referenced specifically in any publications.
Publishing research using ab37720? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"