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Western blot - CD55 antibody (ab52667)
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Product Name
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CD55 antibody
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See all CD55 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to CD55
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Immunogen
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Recombinant fragment: VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREIY , corresponding to amino acids 125-224 of Human CD55
BLAST 'VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREIY ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Isoform 1: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Cell membrane; Lipid-anchor, I-anchor.
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Research areas
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Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins.
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.
his antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, Whole serum Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD55 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD55 antibody images, references, products related to ab52667 and other tools.
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CD55 antibody images:
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 Western blot - CD55 antibody (ab52667)
All lanes : CD55 antibody (ab52667) at 1/1000 dilution
Lane 1 : total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : total protein extract from E coli with 50ng to 500ng of the tagged antigen
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 41 kDa
Note: the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.
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Search PubMed (MEDLINE) for references to CD55
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CD55 antibody - more information
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