Anti-CD57 antibody [TB01] (ab33273)
Key features and details
- Mouse monoclonal [TB01] to CD57
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
Anti-CD57 antibody [TB01]
See all CD57 primary antibodies -
Description
Mouse monoclonal [TB01] to CD57 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, Flow Cytmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD57. Human neuroblastoma cells
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Positive control
- Human tonsil tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
TB01 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/10 - 1/100.
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IHC-P |
1/10 - 1/50.
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining. |
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Flow Cyt |
1/50 - 1/100.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Notes |
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IHC-Fr
1/10 - 1/100. |
IHC-P
1/10 - 1/50. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but sodium citrate buffer may enhance staining. |
Flow Cyt
1/50 - 1/100. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Target
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Relevance
CD57 is a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Is is created by Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (B3GAT1) The CD57 antigen is present on subsets of T cells and natural killer cells. -
Alternative names
- HKN1 antibody
- LEU7 antibody
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab33273 has been referenced in 1 publication.
- Eriksson M et al. Unique phenotype of human uterine NK cells and their regulation by endogenous TGF-beta. J Leukoc Biol 76:667-75 (2004). PubMed: 15178706