![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183) image](#CD59-Primary-antibodies-ab9183-3.jpg)
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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD59 antibody [MEM-43/5]
See all CD59 products (18) ...
Description
Mouse monoclonal [MEM-43/5] to CD59
Specificity
CD59 antigen (human). MEM-43/5 reacts with well defined epitope (around L33) and does not compete with MEM-43 and many other CD59 antibodies
Tested applications
IHC-P, WB, IP, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Thymocytes and T lymphocytes.
Epitope
The antibody MEM-43/5 reacts with well defined epitope around L33 (see Bodian et al)
Positive control
Flow cyt: blood
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified by protein A chromatography.
Clonality
Monoclonal
Clone number
MEM-43/5
Myeloma
unknown
Isotype
IgG2b
Light chain type
unknown
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Immunology >> Innate Immunity >> Complement >> MAC
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183) image](/ps/datasheet/images/9/ab9183/CD59-Primary-antibodies-ab9183-3.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. (CD59 is GPI-anchored, so we recommend to use a laurylmatoside based lysis buffer or triton base buffer (see Bodian et al; 1% Triton X-100, 1 µg/ml leupeptin, 1 µg/ml pepstatin A and 1 mM phenlymethylsulphonyl fluoride in PBS), not NP40.)
IP: Use at an assay dependent dilution.
Flow Cyt: Use a concentration of 1 µg/ml
Target
Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Involvement in disease
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
Sequence similarities
Contains 1 UPAR/Ly6 domain.
Post-translational
modifications
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Cellular localization
Cell membrane. Secreted. Soluble form found in a number of tissues.
Target information above from: UniProt accessionP13987
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
Database links
- Entrez Gene: 966 Human
- Entrez Gene: 12509 Mouse
- Entrez Gene: 333883 Mouse
- Omim: 107271 Human
- SwissProt: P13987 Human
- SwissProt: O55186 Mouse
- SwissProt: P58019 Mouse
- Unigene: 278573 Human
see all
Anti-CD59 antibody [MEM-43/5] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43/5] (ab9183)
IHC image of ab9183 staining CD59 in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab9183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-CD59 antibody [MEM-43/5] (ab9183)
This product has been referenced in:
- Cai Het al. Differential transformation capacity of Src family kinases during the initiation of prostate cancer. Proc Natl Acad Sci U S A 108:6579-84 (2011). WB; Human.Read more (PubMed: 21464326) »
- Renner Bet al. The complement inhibitors Crry and factor H are critical for preventing autologous complement activation on renal tubular epithelial cells. J Immunol 185:3086-94 (2010). Flow Cyt.Read more (PubMed: 20675597) »
See all 5 publications for this product
Publishing research using ab9183? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"