Anti-CD59 antibody [MEM-43] (ab9182)

Product code: 9182
Inquiry: The customer is looking for the secondary anti mouse to stain the cells (ICC/IF) with ab9182. Kindly advise if anti mouse IgG (like ab98794, for instance) can be used or it should be specific anti IgG2a (ab97244). Thank you.

ANSWER:

Thank you for contacting us.
For ab9182 the ab97244 is indeed a compatible secondary antibody that can be used for ICC/IF.
The ab98794 is an IgG antibody which can cross react with IgG2a however because we do not have tested data so we can't 100% guarantee this. Our recommendation is to use the compatible secondary antibody.
Other compatible product is ab97243
As promotion we are giving away protein ladder free. Please click the below link and recommend this to customer;
http://www.abcam.com/index.html?pageconfig=resource&rid=14968
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
http://www.abcam.com/abreviews

I purchased ab9182 which is CD59. I have try dilutions from 1/10 to 1/100 and nothing seems to work. I don't get any staining. Today I tried with placenta, 1/20, antigen retrieval with Tris for 32 min and again nothing. I'm using Benchmark automated stainer. Any suggestions??? The lot # is GR36289-3 and order #1021675. Let me know if you need any other information.

Thanks,

ANSWER:

According to our records, ab9182 was proving difficult to use inIHC and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

No signal in IHC-P on intestine or placental tissue.

ANSWER:

Thank you for calling Abcam.

I am sorry that you were having problems with ab9182 in IHC-P. As we discussed, I am sending you ab9183 as a free of charge replacement and you should have it by 10.30am tomorrow morning. You r new order number is *****.

If there is anything else I can help you with, please let me know.

I purchased ab9182 which is CD59. I have try dilutions from 1/10 to 1/100 and nothing seems to work. I don't get any staining. Today I tried with placenta, 1/20, antigen retrieval with Tris for 32 min and again nothing. I'm using Benchmark automated stainer. Any suggestions??? The lot # is GR36289-3 and order #1021675. Let me know if you need any other information.
Thanks,

ANSWER:

Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

I have read the information you have kindly provided and would like to ask few questions for better understanding of the problem;

- Could you explain the antigen retrieval method bit more? The information I am after is Buffer, buffer ph; hot plate boiling method, microwave boiling method or pressure cooker method.
- Are you suing human placenta sections?
- Which statement you would choose e.g. high background, no staining or light staining.
- What was the secondary antibody e.g species, type, isotype, dilution etc?
- Have you used same secondary antibody before? Is it true the anomaly is not due to secondary antibody?
- Does the detection system works fine? Have you tried using it with other antibodies and tissue sections?
- Could you send us an image?
- How the antibody was stored since ordering?
- Have you preformed any other troubleshooting than changing dilutions?

In order to help you further with this antibody I will look forward to hearing from you .

I was wondering whether these would crossreact with mouse cd59. Thank you.

ANSWER:

These two monoclonals (ab9182, ab9183) have not been tested for cross reactivity with mouse CD59.