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Flow Cytometry - CD59 antibody [MEM-43] (ab9182)
![Flow Cytometry - CD59 antibody [MEM-43] (ab9182)](/ps/datasheet/images/9/ab9182/CD59-Primary-antibodies-ab9182-2.jpg)
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Product Name
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CD59 antibody [MEM-43]
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See all CD59 antibodies (17)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MEM-43] to CD59
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Immunogen
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Thymocytes and T lymphocytes.
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Reacts with
(species key)
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Hu
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Specificity
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CD59 antigen (human).
MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop.
MEM-43 reacts with well defined epitope (WhO, P-53).
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Tested applications
(see key)
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Flow Cyt, FuncS, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
FuncS: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution PubMed: 17911601.
Flow Cyt: Use at an assay dependant concentration.
FuncS: Use at an assay dependant dilution. MEM-43 inhibits the complement-protecting activity of CD59.
IP: Use at an assay dependant concentration.
WB: Use at an assay dependant concentration. Use under non-reducing conditions.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH6.0 is recommended for this purpose.
IHC-Fr: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell membrane; Lipid-anchor, GPI-anchor. Secreted
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Research areas
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Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Immunology >> Innate Immunity >> Complement >> MAC
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Relevance
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CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MEM-43
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Isotype
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IgG2a
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified by protein A chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD59 antibody [MEM-43] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD59 antibody [MEM-43] images, references, products related to ab9182 and other tools.
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CD59 antibody [MEM-43] images:
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 Flow Cytometry - CD59 antibody [MEM-43] (ab9182)
ab9182 staining CD59 in Human fibrosarcoma (HT1080) by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody,10 µg/ml in PBS, for 1 hour at 20°C. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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References for CD59 antibody [MEM-43] (ab9182)
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This product has been used in: (two most recent references)
Kolev MV et al. Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration. Pharmacogenomics J 10:12-9 (2010). Flow Cyt; Human. PubMed: 19884909
At-Slimane T et al. Basolateral internalization of GPI-anchored proteins occurs via a clathrin-independent flotillin-dependent pathway in polarized hepatic cells. Mol Biol Cell 20:3792-800 (2009). ICC/IF; Human. PubMed: 19605558
See all 15 publication references for this product.
If you publish research using ab9182 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD59
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CD59 antibody [MEM-43] - more information
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Customer reviews (feedback) regarding CD59 antibody [MEM-43] |
Customer FAQs regarding CD59 antibody [MEM-43] |
Protocols for CD59 antibody [MEM-43] |
Price and availability of products related to CD59 antibody [MEM-43] |
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CD59 antibody [MEM-43] - related products:
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CD59 antibody [MEM-43] - other tools:
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Contact Abcam with a Technical Enquiry about CD59 antibody [MEM-43] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - CD59 antibody [MEM-43] (ab9182)](/index.html?datasheet=9182#CD59-Primary-antibodies-ab9182-2.jpg)
