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Products:Immunology >> Cell Type Markers >> CD >> Non-lineage
Overview
Product name
Anti-CD59 antibody
See all CD59 products (18) ...
Description
Rabbit polyclonal to CD59
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human CD59.
(Peptide available as ab69083.)
Positive control
This antibody gave a positive signal in the following Human lysates: Spleen Tissue, Colon Tissue, Brain Tissue, K562 Whole Cell, THP1 Whole Cell, U937 Whole Cell, HL60 Whole Cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Innate and adaptive immunity
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Immunology >> Innate Immunity >> Complement >> MAC
Immunology >> Cell Type Markers >> CD >> Non-lineage
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Western blot - CD59 antibody (ab69084)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 22 kDa (predicted molecular weight: 14 kDa).
Target
Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Involvement in disease
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
Sequence similarities
Contains 1 UPAR/Ly6 domain.
Post-translational
modifications
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Cellular localization
Cell membrane. Secreted. Soluble form found in a number of tissues.
Target information above from: UniProt accessionP13987
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
Database links
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
Anti-CD59 antibody images:
Western blot - CD59 antibody (ab69084)
All lanes : Anti-CD59 antibody (ab69084) at 1 µg/ml
Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
Lane 2 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 5 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 6 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
Lane 7 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
The 22 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to CD59.
References for Anti-CD59 antibody (ab69084)
ab69084 has not yet been referenced specifically in any publications.
Publishing research using ab69084? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"