Overview

  • Product nameAnti-CD6 antibody [EPR4057]See all CD6 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR4057] to CD6
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human CD6.

  • Positive control
    • WB: Jurkat, Human tonsil, PBMC and HuT-78 lysates IHC-P: Human tonsil and Human lymphocytes infiltrating colon tissues
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab109217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 90-130 kDa (predicted molecular weight: 72 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionInvolved in cell adhesion. Binds to CD166.
    • Tissue specificityExpressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
    • Sequence similaritiesContains 3 SRCR domains.
    • Post-translational
      modifications
      After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
      Contains intrachain disulfide bond(s).
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    Anti-CD6 antibody [EPR4057] images

    • All lanes : Anti-CD6 antibody [EPR4057] (ab109217) at 1/1000 dilution

      Lane 1 : Jurkat lysates
      Lane 2 : Human tonsil lysates
      Lane 3 : PBMC lysates
      Lane 4 : HuT-78 lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 72 kDa
      Observed band size : 90-130 kDa (why is the actual band size different from the predicted?)
    • ab109217, at a 1/100 dilution, staining CD6 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • ab109217, at a 1/100 dilution, staining CD6 in paraffin embedded Human lymphocytes infiltrating colon tissue by Immunohistochemistry.
    • ab109217 showing negative staining in Skeletal muscle tissue.

    • ab109217 showing negative staining in Normal brain tissue.

    • ab109217 showing negative staining in Normal kidney tissue.

    • ab109217 showing negative staining in Normal uterus tissue.

    References for Anti-CD6 antibody [EPR4057] (ab109217)

    ab109217 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"