CD62E Human ELISA Kit (ab45915)

Overview

• Product nameCD62E Human ELISA KitSee all CD62E kits ...
• Detection methodColorimetric
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	12	31.55ng/ml	0.272	0.86%
  B	12	4.22ng/ml	0.174	4.11%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	32	31.34ng/ml	0.477	1.52%
  B	32	4.18ng/ml	0.266	6.36%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 0.5 ng/ml
• Range
  1 ng/ml - 32 ng/ml
• Assay time
  2h 10m
• Assay durationMultiple steps standard assay
• Species reactivity
  Reacts with: Human
  
• Product overview

  INTENDED USE

  The Human CD62E ELISA is to be used for the in-vitro quantitative determination of soluble Endothelial Leucocyte Adhesion Molecule ELAM1 (CD62E) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD62E. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  PRINCIPLE OF THE METHOD

  The CD62E Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD62E has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62E concentrations and unknowns are pipetted into these wells.

  During the first incubation, the CD62E antigen is added to wells. After washing, a biotinylated monoclonal antibody specific for CD62E is incubated. Then the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all unbound enzyme, a substrate solution which acts on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD62E present in the samples.

   

  Recognizes both natural and recombinant human CD62E
  
  

• Notes

  Safety Information:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsSandwich ELISA more details
• PlatformMicroplate

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  10X Standard Diluent Buffer	Black	1 x 25ml
  200X Washing Buffer	White	2 x 10ml
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Biotinylated anti-CD62E antibody	Red	2 x 0.4ml
  CD62E Control	Silver	4 vials
  CD62E precoated microtiter plates		8 units
  CD62E Standard	Yellow	4 vials
  Chromogen TMB		1 x 24ml
  H2SO4: Stop Reagent	Black	2 x 11ml
  HRP Diluent	Red	1 x 23ml
  Stop Reagent	Black	2 x 11ml
  Streptavidin-HRP		4 x 5µl

• Research Areas
  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • CD markers ELISA kits

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Developmental proteins ELISA kits

  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Cell Adhesion Molecules
  • Vascular

  • Immunology
  • Cell Type Markers
  • CD
  • Adhesion

Applications

CD62E Human ELISA Kit images

• 

  ELISA - Human CD62E ELISA kit (ab45915)
  Typical CD62E standard curve ranging from 1 to 32 ng/mL

Protocols

• Human CD62E ELISA Kit