CD62E Human ELISA Kit (ab100512)
- Product nameCD62E Human ELISA KitSee all CD62E kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range24.69 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.39 83% - 103% Serum 93.75 83% - 102% Plasma 95.38 84% - 105%
- Species reactivityReacts with: Human
- Product overview
ab100512, a Human CD62E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD62E in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer, For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml CD62E Microplate (12 strips x 8 wells) coated with anti-human CD62E 1 unit CD62E Standard Human Recombinant 2 vials Detection Antibody CD62E: biotinylated anti-human CD62E 2 vials HRP-Streptavidin Concentrate 900x concentrated HRP-conjugated streptavidin 1 x 200µl Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
- CD62 antigen-like family member ECD62ECD62E antigen
- E selectin E-selectinELAM ELAM-1ELAM1Endothelial leukocyte adhesion molecule 1ESEL LECAM2Leukocyte endothelial cell adhesion molecule 2Leukocyte-endothelial cell adhesion molecule 2LYAM2_HUMANSele
Our Abpromise guarantee covers the use of ab100512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CD62E Human ELISA Kit (ab100512)
ab100512 has not yet been referenced specifically in any publications.