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CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47360)

Overview

  • Product nameCD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates)See all CD62E kits ...
  • Tests
    10 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeELIPAIR
  • Sensitivity
    < 0.5 ng/ml
  • Range
    1 ng/ml - 32 ng/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    Intended Use

    This Eli-pair set is intended to measure human ELAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Principle of Method

    The ELAM-1 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human CD62E

  • Tested applicationsELISA more details

Properties

  • RelevanceCD62E is expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by CD62E is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids).
  • Cellular localizationMembrane; single-pass type I membrane protein
    • Alternative names
        ELAMELAM1ESEL
        LECAM2leukocyte endothelial cell adhesion molecule 2SELESelectin E (endothelial adhesion molecule 1)
      see all
  • Database links
  • Applications

    Our Abpromise guarantee covers the use of ab47360 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA
  • Application notesELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) images

    • CD62E Standard curve ranging from 1 to 32 ng/mL

    References for CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47360)

    ab47360 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"