CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47360)
Overview
- Product nameCD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates)See all CD62E kits ...
- Tests10 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeELIPAIR
- Sensitivity< 0.5 ng/ml
- Range1 ng/ml - 32 ng/ml
- Species reactivityReacts with: Human
- Product overview
Intended Use
This Eli-pair set is intended to measure human ELAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Principle of Method
The ELAM-1 Eli-pair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human CD62E
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 10 x 96 tests Capture Ab B-P7 2 x 0.25ml CD62E Eli-standard 10 vials Detection biotinylated antibody B-S3 (Reconstitute with 2x 0.55 ml PBS 0.1% Azide w/v or another preservative. ) 2 x 0.55ml Ready-to-use TMB 4 vials Streptavidin-HRP 2 vials - Research Areas
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Alternative names
- ELAMELAM1ESEL
- LECAM2leukocyte endothelial cell adhesion molecule 2SELESelectin E (endothelial adhesion molecule 1)
see all
- Entrez Gene: 6401 Human
- SwissProt: P16581 Human
Applications
Our Abpromise guarantee covers the use of ab47360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) images
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CD62E Standard curve ranging from 1 to 32 ng/mL
Protocols
References for CD62E Human Elipair Kit (Reagents for 10 x 96 Well Plates) (ab47360)
ab47360 has not yet been referenced specifically in any publications.
