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Product name
Anti-CD62E antibody [ENA1] (Biotin)
See all CD62E products (18) ...
Description
Mouse monoclonal [ENA1] to CD62E (Biotin)
Conjugation
Biotin
Specificity
This antibody reacts with human endothelial cells activated with TNF, IL1 or endotoxin. It was found to react also with cells transfected with the the E-selectin gene.
Tested applications
IHC-Fr, ICC/IFmore details
Cross reactivity
Reacts with
Human, Chimpanzee, Baboon, Cynomolgus Monkey, Rhesus monkey
Immunogen
Tissue / cell preparation containing human CD3
Positive control
Endothelial cells or a small fresh skin biopsy after incubation with TNF, IL1 or LPS.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS
Concentration
Concentration information loading...
Primary antibody notes
This antibody is useful for the staining of CD62E expressing endothelial cells. This antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
Clonality
Monoclonal
Clone number
ENA1
Isotype
IgG1
Research areas
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Endothelial Cells
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
ICC/IF
Application notes
ICC/IF [fixed with paraformaldehyde]: Use at an assay dependent dilution.
IHC-Fr [fixed with acetone, followed by incubation for 10 min in chloroform]: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionP16581
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- E selectin antibody
- CD 62E antibody
- CD26E antibody
see all
Database links
- Entrez Gene: 6401 Human
- Omim: 131210 Human
- SwissProt: P16581 Human
- Unigene: 82848 Human
References for Anti-CD62E antibody [ENA1] (Biotin) (ab36688)
This product has been referenced in:
- Leeuwenberg JF et al. Adhesion of polymorphonuclear cells to human endothelial cells. Adhesion-molecule-dependent, and Fc receptor-mediated adhesion-molecule-independent mechanisms. Clin Exp Immunol 81:496-500 (1990).Read more (PubMed: 1697800) »
- Leeuwenberg JF et al. Functional polymorphism of IgG FcRII (CD32) on human neutrophils. Immunology 71:301-4 (1990).Read more (PubMed: 2146215) »
See all 3 publications for this product
Publishing research using ab36688? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"