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Overview

  • Product nameCD62L Human ELISA KitSee all CD62L kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 15 28ng/ml 1.3 4.6%
    Sample B 19 9ng/ml 0.5 5.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 ng/ml
  • Range
    1.6 ng/ml - 60 ng/ml
  • Assay time
    1h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human CD62L ELISA is to be used for the in-vitro quantitative determination CD62L in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD62L. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal

    The CD62L Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations and unknowns are pipetted into these wells. During the first incubation, the CD62L antigen and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD62L present in the samples.

     

    Recognizes both natural and recombinant human soluble CD62L

    Range: 1.6 ng/ml - 60 ng/ml

    Sensitivity <1 ng/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceCD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
    • Alternative names
        A.11 AI528707CD62 antigen ligand
        CD62 antigen-like family member LCD62L antigengp90 MEL hLHRcIgA nephropathy, susceptibility to, included L Selectin L selectin precursorLAM 1LAM1 LECAM1 Leu 8Leu8Leukocyte adhesion molecule 1 Leukocyte endothelial cell adhesion molecule 1Leukocyte surface antigen Leu 8Leukocyte surface antigen Leu8 Leukocyte-endothelial cell adhesion molecule 1 LNHRLSELLy-22 Ly-m22 Lyam 1LYAM1 Lymph node homing receptor Lymphocyte adhesion molecule 1Lymphocyte antigen 22 Lymphocyte surface MEL-14 antigen PLNHR Selectin L Selectin L (lymphocyte adhesion molecule 1)Selectin, lymphocyte SELLTQ1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45917 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD62L Human ELISA Kit images

      • ELISA - Human CD62L ELISA Kit (ab45917)
        ELISA - Human CD62L ELISA Kit (ab45917)
        Typical CD62L standard curve ranging from 1.9 to 60 ng/ml.

      References for CD62L Human ELISA Kit (ab45917)

      This product has been referenced in:
      • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

      See 1 Publication for this product

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      Dear Abcam,
      Thank you the discount code.
      I will contact local distributor in Thailand for ordering.

      Thank you for your order. I wish you the best of luck with your research.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"