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Products:Cardiovascular >> Blood >> Other

Anti-CD62L antibody [MEL-14] (PE/Cy7 ®) (ab25569)

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Overview

Product name

Anti-CD62L antibody [MEL-14] (PE/Cy7 ®)
See all CD62L products (34) ...

Description

Rat monoclonal [MEL-14] to CD62L (PE/Cy7 ®)

Conjugation

PE/Cy7 ®

Specificity

ab25569 recognises CD62L.

Tested applications

Functional Studies, Flow Cyt, IHC-Fr, IP, Blocking, Inhibition Assaymore details

Cross reactivity

Reacts with

Mouse

Immunogen

Tissue / cell preparation: C3H/eb cloned mouse B lymphoma 38C-13.

General notes

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Form

Liquid

Storage instructions

Store at +4°C in the dark. Do not freeze.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, PBS and stabilizing agent.

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

MEL-14

Isotype

IgG2a

Light chain type

kappa

Research areas

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Immunology >> Adaptive Immunity >> T Cells >> CD
Cardiovascular >> Blood >> Other

Applications

Show applications key

Our Abpromise guarantee covers the use of ab25569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution. (Note: ab25569 can be used for cytotoxicity studies.)

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    Flow Cyt

      See more...Read more →

    Flow Cyt: Use 0.1µg for 106 cells.

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    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

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    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    Blocking

     BL: Use at an assay depe...Read more →

    BL: Use at an assay dependent dilution. (Ab25569 can be used to block in vitro binding of lymphocytes to peripheral lymph node HEV.)

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    Inhibition Assay

     Inhib: Use at an assay d...Read more →

    Inhib: Use at an assay dependent dilution. (Ab25569 inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.)

Target

Function

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

Tissue specificity

Expressed in B cell lines and T lymphocytes.

Sequence similarities

Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains.

Cellular localization

Membrane.

Target information above from: UniProt accessionP14151 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • A.11 antibody
  • AI528707 antibody
  • CD62 antigen ligand antibody
  • CD62 antigen-like family member L antibody
  • CD62L antibody
  • gp90 MEL antibody
  • gp90-MEL antibody
  • hLHRc antibody
  • IgA nephropathy, susceptibility to, included antibody
  • L Selectin antibody
  • L-selectin antibody
  • LAM-1 antibody
  • LAM1 antibody
  • LECAM1 antibody
  • LEU8 antibody
  • Leukocyte adhesion molecule 1 antibody
  • Leukocyte surface antigen Leu-8 antibody
  • Leukocyte surface antigen Leu8 antibody
  • Leukocyte-endothelial cell adhesion molecule 1 antibody
  • LNHR antibody
  • LSEL antibody
  • Ly-22 antibody
  • Ly-m22 antibody
  • Lyam-1 antibody
  • LYAM1 antibody
  • LYAM1_HUMAN antibody
  • Lymph node homing receptor antibody
  • Lymphocyte adhesion molecule 1 antibody
  • Lymphocyte antigen 22 antibody
  • Lymphocyte surface MEL-14 antigen antibody
  • MEL-14 antibody
  • Pln homing receptor antibody
  • PLNHR antibody
  • Selectin L antibody
  • Selectin, lymphocyte antibody
  • SELL antibody
  • TQ1 antibody
see all

Database links

References for Anti-CD62L antibody [MEL-14] (PE/Cy7 ®) (ab25569)

This product has been referenced in:

  • Jones Met al. IL-10 Restricts Memory T Cell Inflation during Cytomegalovirus Infection. J Immunol 185:3583-92 (2010). Flow Cyt; Mouse.Read more (PubMed: 20713884) »

See 1 publication for this product

Publishing research using ab25569? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"