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Overview

  • Product nameCD62L protein (Fc Chimera)See all CD62L proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 1-343 of Human CD62L fused to the Fc region of human IgG1 (aa 93-330), expressed in modified human 293 cells. Swissprot ID = P14151.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical Sequence: WTYHYSEKPMNWQRARRFCRDNYTDLVAI QNKAEIEYLEKTLPFSRSYYWIGIRKIGGI WTWVGTNK SLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKDAGKWNDD ACHKLKAAL CYTASCQPWSCSGHGECVEIINNYTCNCD VGYYGPQCQFVIQCEPLEAPELGTMDCTH PLGNFSFSS QCAFSCSEGTNLTGIEETTCGPFGNWSSPEPTCQVIQCEP LSAPDLGIMN CSHPLASFSFTSACTFICSEGTELIGKK KTICESSGIWSNPSPICQKLDKSFSMIKEGDWI PKVDK KVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVS HEDPEVKFNWYVDGVEVHNAKTKP REEQYNSTYRVVSVLTVLHQDWLNGKEYKCRVS NKALP APIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLV KGFYPSDIAVEWESN GQPENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSL SLSP GK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83824 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Molecular Mass: ab83824 migrates as a broad band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified CD62L-Fc Chimera that has a predicted molecular mass of 59.9kDa.

    pI: ab83824 separates into a number of isoforms with a pI between 4.6 and 6.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified CD62L-Fc Chimera that has a predicted pI of 5.92.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        A.11AI528707CD62 antigen ligand
        CD62 antigen-like family member LCD62Lgp90-MELIgA nephropathy, susceptibility to, includedL SelectinL-selectinLAM-1LAM1LECAM1LEU8Leukocyte adhesion molecule 1Leukocyte surface antigen Leu-8Leukocyte-endothelial cell adhesion molecule 1LnhrLSELLy-22Ly-m22Lyam-1LYAM1LYAM1_HUMANLymph node homing receptorLymphocyte adhesion molecule 1Lymphocyte antigen 22Lymphocyte surface MEL-14 antigenMEL-14Pln homing receptorPLNHRSelectin LSelectin, lymphocyteSELLTQ1
      see all
  • FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificityExpressed in B cell lines and T lymphocytes.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P14151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CD62L protein (Fc Chimera) images

    • Functional Studies - CD62L protein (Fc Chimera) (ab83824)
      Functional Studies - CD62L protein (Fc Chimera) (ab83824)
      Densitometry of protein isoforms visualised by 2-DE.
      The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
      The triangle indicates the theoretical MW and pI of the protein.
    • SDS-PAGE - CD62L protein (Fc Chimera) (ab83824)
      SDS-PAGE - CD62L protein (Fc Chimera) (ab83824)
      1D SDS-PAGE of ab83824 before and after treatment with glycosidases to remove oligosaccharides.
      Lane 1: ab83824
      Lane 2: ab83824 treated with PNGase F to remove potential N-linked glycans
      10 µg protein loaded per lane; Deep Purple™ stained.
      Drop in MW after treatment with PNGase F indicates presence of N-linked glycans.

    References for CD62L protein (Fc Chimera) (ab83824)

    ab83824 has not yet been referenced specifically in any publications.

    Publishing research using ab83824 ? Please let us know so that we can cite the reference in this datasheet

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