CD62L protein (Fc Chimera) (ab83824)
Constituents: 10% Trehalose, 1% Human serum albumin, PBS
Our Abpromise guarantee covers the use of ab83824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Molecular Mass: ab83824 migrates as a broad band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified CD62L-Fc Chimera that has a predicted molecular mass of 59.9kDa.
pI: ab83824 separates into a number of isoforms with a pI between 4.6 and 6.5 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified CD62L-Fc Chimera that has a predicted pI of 5.92.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- A.11AI528707CD62 antigen ligand
- CD62 antigen-like family member LCD62Lgp90-MELIgA nephropathy, susceptibility to, includedL SelectinL-selectinLAM-1LAM1LECAM1LEU8Leukocyte adhesion molecule 1Leukocyte surface antigen Leu-8Leukocyte-endothelial cell adhesion molecule 1LnhrLSELLy-22Ly-m22Lyam-1LYAM1LYAM1_HUMANLymph node homing receptorLymphocyte adhesion molecule 1Lymphocyte antigen 22Lymphocyte surface MEL-14 antigenMEL-14Pln homing receptorPLNHRSelectin LSelectin, lymphocyteSELLTQ1
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains.
CD62L protein (Fc Chimera) images
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
1D SDS-PAGE of ab83824 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83824
Lane 2: ab83824 treated with PNGase F to remove potential N-linked glycans
10 µg protein loaded per lane; Deep Purple™ stained.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans.
References for CD62L protein (Fc Chimera) (ab83824)
ab83824 has not yet been referenced specifically in any publications.