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Overview

  • Product nameCD62P Human ELISA KitSee all CD62P kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 20 pg/ml
  • Range
    0.041 ng/ml - 30 ng/ml
  • Recovery

    72 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 72.51 63% - 85%
    Serum 74.98 65% - 86%
    Plasma 68.29 60% - 78%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100631 Human CD62P ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody P-Selectin (CD62P): biotinylated anti-human P-Selectin (CD62P) 2 vials
    HRP-Streptavidin Concentrate 800x concentrated HRP-conjugated streptavidin 1 x 200µl
    P Selectin Microplate (12 strips x 8 wells) coated with anti-human P-Selectin 1 unit
    P Selectin Standard Human(recombinant) 2 vials
  • Research Areas
  • FunctionCa(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificityStored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in diseaseDefects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P16109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Antigen CD62CD62CD62 antigen like family member P
        CD62 antigen-like family member PCD62PFLJ45155GMP 140GMP-140GMP140Granule membrane protein 140Granule membrane protein 140kDaGranulocyte membrane proteinGRMPLECAM3Leukocyte endothelial cell adhesion molecule 3 Leukocyte-endothelial cell adhesion molecule 3LYAM3_HUMANP selectinP-selectinPADGEMPlatelet activation dependent granule external membrane proteinPlatelet activation dependent granule-external membrane proteinPlatelet alpha granule membrane proteinPSELSelectin PSelectin P (granule membrane protein 140kDa antigen CD62)SELP
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100631 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CD62P Human ELISA Kit images

      • ELISA - CD62P Human ELISA Kit (ab100631)
        ELISA - CD62P Human ELISA Kit (ab100631)
      • ELISA - CD62P Human ELISA Kit (ab100631)
        ELISA - CD62P Human ELISA Kit (ab100631)

      Protocols

      References for CD62P Human ELISA Kit (ab100631)

      ab100631 has not yet been referenced specifically in any publications.

      Publishing research using ab100631 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100631 Submit a Question for ab100631

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

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      Votes: Highest First

      Thanks for your response. In reply to both of your emails (CCE3286260 and CCE3288654) I would like to answer you this. CCE3286260 1. Please note that diluent B should be used to dilute HRP-streptavidin As mentioned on the telephone, this was not ...

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      Thank you for your reply. I agree with your points. The protocol did not specify which diluent to use and this is likely to be the cause of the problem. I was hoping that you might have some reagents left to try the procedure again with the changes sug...

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      No signal in plate (standards and samples). Testing plasma. Using diluent A in steps 4,6 & 7. Standard diluted as described in protocol 30ng to 0. Plate incubated with samples at room temperature for 2.5 hr.  

      Thank you for your patience. I received further information from the lab regarding component dilution. - Briefly spin the HRP-Streptavidin concentrate vial and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be di...

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      I am observing weak signal with this kit.

      I am very sorry for the delay. I received the following information from the lab.Please note that diluent B should be used to dilute HRP-streptavidin: - Detection Antibody – once reconstituted, this must be used within a couple of days. We provide ...

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      No signal in plate (standards and samples). Testing plasma. Using diluent A in steps 4,6 & 7. Standard diluted as described in protocol 30ng to 0. Plate incubated with samples at room temperature for 2.5 hr.  

      Thank you for your patience. I have just received this reply for the lab, would it be possible for you to answer their questions? (1) After reviewing the data I noticed there were 90 samples and 6 standards. The sample identifications just say “sa...

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      No signal in plate (standards and samples). Testing plasma. Using diluent A in steps 4,6 & 7. Standard diluted as described in protocol 30ng to 0. Plate incubated with samples at room temperature for 2.5 hr.  

      Thank you for your patience. The lab have requested some additional information to investigate the problem in more detail. How was the kit stored and for how long? Type of sample, if diluted what dilution was used? How were the samples prepared an...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"