CD62P antibody [AK-6] (ab6632)

Product Name 

CD62P antibody [AK-6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AK-6] to CD62P

Reacts with 
(species key)

Hu, RMk

Specificity 

MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/10 - 1/100.
IHC-P: 1/500 - 1/2000.
IHC-Fr: 1/1000 - 1/5000.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets

Relevance 

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AK-6

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for CD62P antibody [AK-6] images, references, products related to ab6632 and other tools.

CD62P antibody [AK-6] images:

References for CD62P antibody [AK-6] (ab6632)

  This product has been used in:

Fleming JN et al. Capillary regeneration in scleroderma: stem cell therapy reverses phenotype? PLoS ONE 3:e1452 (2008). IHC-P; Human. PubMed: 18197262

If you publish research using ab6632 please let us know so that we can cite the reference on this datasheet.

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