CD62P antibody [Psel.KO.2.7] (ab54427)

Product Name 

CD62P antibody [Psel.KO.2.7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Psel.KO.2.7] to CD62P

Immunogen 

CD62P transfected 300.19 cells (Human)

Reacts with 
(species key)

Hu, Ms, Rat, Cat, Cow, Goat, Hrs, Shp

Specificity 

This antibody recognizes the CD62P cell surface antigen

Tested applications 
(see key)

Flow Cyt, IHC-Fr, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/25 - 1/50. Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Thrombin activated human platelets

Cellular localization 

Membrane; single pass type I membrane protein.

Research areas 

Immunology >> Adaptive Immunity >> Regulatory T Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets

Relevance 

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Psel.KO.2.7

Myeloma 

NS1

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from tissue culture supernatant

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Shelf Life - 18 months, product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

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