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Products:Cardiovascular >> Blood >> Platelets

Anti-CD62P antibody (ab59738)

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1 question for ab59738

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Question 1

Thursday 27-May-2010

Can you determine if it binds to the extracellular domain epitope of P-selectin on rat endothelial cells?

Thank you very much!

ANSWER:

  

Thank you for your inquiry.

Unfortunately this antibody is very unlikely to recognize the extracellular domain of the rat P-selectin, as it has been raised against a peptide surrounding amino acid 756. Indeed accordingly to SwissProt, amino acids 734 to 768 are the cytoplasmic domain of the rat P-selectin. http://www.uniprot.org/uniprot/P98106

We do have however two other antibodies against P-selectin which have been tested successfully to recognize the cell surface P-selectin, ab54427 and ab54426, as they work in flow cytometry without permeabilization.

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