Anti-CD63 antibody [MEM-259] (ab8219)
Key features and details
- Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies -
Description
Mouse monoclonal [MEM-259] to CD63 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD63. HPB-ALL T cell line
-
Positive control
- IHC: Human spleen sections; ICC: Human HeLa cells, primary fibroblasts and skin fibroblasts tissues; Flow cyt: Human blood samples.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-259 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt | (2) |
Use a concentration of 2 µg/ml.
Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC | (1) |
Use at an assay dependent concentration.
|
IHC-P | (1) |
Use a concentration of 10 µg/ml.
|
Notes |
---|
Flow Cyt
Use a concentration of 2 µg/ml. Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
Target
-
Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
-
Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
-
Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
-
Immunohistochemistry staining of human spleen (paraffin sections) using ab8219.
-
Immunocytochemistry staining of human skin fibroblasts with ab8219 (green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).
-
ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.
-
Immunocytochemistry staining of CD63 in human HeLa cell line using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
-
Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml).
-
Immunocytochemistry staining of CD63 in human primary fibroblasts using ab8219 (green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (94)
ab8219 has been referenced in 94 publications.
- Park SJ et al. Vinexin contributes to autophagic decline in brain ageing across species. Cell Death Differ 29:1055-1070 (2022). PubMed: 34848853
- Krishn SR et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the αVβ6 integrin and inhibits adhesion and migration of recipient prostate cancer cells. Cancer Biol Ther 23:173-185 (2022). PubMed: 35188070
- Governa V et al. Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35217608
- Solvik TA et al. Secretory autophagy maintains proteostasis upon lysosome inhibition. J Cell Biol 221:N/A (2022). PubMed: 35446347
- Park SS et al. Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1. Cancers (Basel) 14:N/A (2022). PubMed: 36077620