![Immunocytochemistry/ Immunofluorescence - CD63 antibody [MEM-259] (ab8219) image](#CD63-Primary-antibodies-ab8219-3.jpg)
![Flow Cytometry - CD63 antibody [MEM-259] (ab8219) image](#CD63-Primary-antibodies-ab8219-4.jpg)
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Products:Cardiovascular >> Blood >> Platelets
Overview
Product name
Anti-CD63 antibody [MEM-259]
See all CD63 products (22) ...
Description
Mouse monoclonal [MEM-259] to CD63
Specificity
CD63 antigen (human)
Tested applications
Flow Cyt, IP, IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Tissue / cell preparation (Human). (HPB-ALL T cell line)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
MEM-259
Isotype
IgG1
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use a concentration of 2 µg/ml(Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.)
IP: Use at an assay dependent concentration.
IHC-P: Use a concentration of 10 µg/ml
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent dilution. Use under non reducing condition.
Target
Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Sequence similarities
Belongs to the tetraspanin (TM4SF) family.
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
Target information above from: UniProt accessionP08962
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
Anti-CD63 antibody [MEM-259] images:
Immunocytochemistry/ Immunofluorescence - CD63 antibody [MEM-259] (ab8219)
![Immunocytochemistry/ Immunofluorescence - CD63 antibody [MEM-259] (ab8219)](/ps/datasheet/images/8/ab8219/CD63-Primary-antibodies-ab8219-3.jpg)
ab8219 staining CD63 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody which was used diluted to 10 µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
This image is a courtesy of Anonymous Abreview
Flow Cytometry - CD63 antibody [MEM-259] (ab8219)
![Flow Cytometry - CD63 antibody [MEM-259] (ab8219)](/ps/datasheet/images/8/ab8219/CD63-Primary-antibodies-ab8219-4.jpg)
ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-CD63 antibody [MEM-259] (ab8219)
This product has been referenced in:
- Duchez Set al. Reciprocal polarization of T and B cells at the immunological synapse. J Immunol 187:4571-80 (2011).Read more (PubMed: 21930964) »
- Ashiru Oet al. Natural killer cell cytotoxicity is suppressed by exposure to the human NKG2D ligand MICA*008 that is shed by tumor cells in exosomes. Cancer Res 70:481-9 (2010). WB; Human.Read more (PubMed: 20068167) »
See all 2 publications for this product
Publishing research using ab8219? Please let us know so that we can cite the reference in this datasheet
![CD63 antibody [MEM-259] for Flow Cyt in Human (8219)](/ps/datasheet/images/8/ab8219/CD63-Primary-antibodies-ab8219-2.jpg)
![CD63 antibody [MEM-259] for ICC/IF in Human (8219)](/ps/datasheet/images/8/ab8219/CD63-Primary-antibodies-ab8219-1.gif)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"