CD63 antibody [NK1/C3] (ab1318)

Product Name 

CD63 antibody [NK1/C3]

Product type 

Primary antibodies

Description 

Mouse monoclonal [NK1/C3] to CD63

Immunogen 

Smooth plasma membrane fraction of MeWo cells.

Reacts with 
(species key)

Hu

Specificity 

This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method.
However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user.
High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining.
We suggest an incubation period of 30 minutes at room temperature.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Note= Also found in Weibel-Palade bodies of endothelial cells

Research areas 

Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Immunology >> Cell Type Markers >> CD >> Platelets
Cardiovascular >> Blood >> Platelets

Relevance 

CD63 (Melanoma 1 antigen) is a glycoprotein of 40-60 kDa belonging to tetraspans family (TM4SF). CD63 is intracellular lysosomal, endosomal and granule protein that is translocated to cell surface upon activation of platelets, endothelial cells, and granulocytes (activation marker). CD63 is also expressed on monocytes, macrophages and endothelium. The cellular function has not been exactly elucidated. CD63 is thought to be associated with the early stages of melanoma tumor progression (in regulation of motility and adhesion of melanoma cells).

CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

NK1/C3

Isotype 

IgG1

Light chain type 

kappa

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for CD63 antibody [NK1/C3] images, references, products related to ab1318 and other tools.

CD63 antibody [NK1/C3] images:

References for CD63 antibody [NK1/C3] (ab1318)

  This product has been used in: (two most recent references)

Turner NA et al. Endothelial cell ADAMTS-13 and VWF: production, release, and VWF string cleavage. Blood 114:5102-11 (2009). ICC/IF; Human. PubMed: 19822897

Irelan JT et al. A role for IkappaB kinase 2 in bipolar spindle assembly. Proc Natl Acad Sci U S A 104:16940-5 (2007). PubMed: 17939994

See all 7 publication references for this product.


If you publish research using ab1318 please let us know so that we can cite the reference on this datasheet.

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