Anti-CD63 antibody (ab68418)
Key features and details
- Rabbit polyclonal to CD63
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-CD63 antibody
See all CD63 primary antibodies -
Description
Rabbit polyclonal to CD63 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD63 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93074) -
Positive control
- This antibody gave a positive signal in Human platelet and HepG2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24,43 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24,43 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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All lanes : Anti-CD63 antibody (ab68418) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Wild-type HAP1 Breferldin A treated whole cell lysate
Lane 3 : CD63 knockout HAP1 whole cell lysate
Lane 4 : CD63 knockout HAP1 Breferldin A treated whole cell lysate
Lane 5 : HL60 whole cell lysate
Lane 6 : Human platelets whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 6: Merged signal (red and green). Green - ab68418 observed at 26 kDa. Red - loading control, ab8245, observed at 38 kDa.
ab68418 was shown to recognize CD63 in wild-type HAP1 as signal was lost at the expected MW in CD63 knockout HAP1 cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD63 knockout samples were subjected to SDS-PAGE. Ab68418 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-CD63 antibody (ab68418) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68418 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
CD63 has a predicted molecular weight of 25 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This is consistent with the literature which describes a band between 30-60 kDa in human platelets(PMID:1993697).
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Anti-CD63 antibody (ab68418) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Additional bands at: 145 kDa, 42 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThe 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human CD63 antigen.
Datasheets and documents
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SDS download
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Datasheet download
References (105)
ab68418 has been referenced in 105 publications.
- Guo M et al. Adipose-derived stem cell-derived extracellular vesicles inhibit neuroblastoma growth by regulating GABBR1 activity through LINC00622-mediated transcription factor AR. J Leukoc Biol 111:19-32 (2022). PubMed: 34448502
- Shang F et al. Endothelial MicroRNA-483-3p Is Hypertension-Protective. Oxid Med Cell Longev 2022:3698219 (2022). PubMed: 35222797
- Hareendran S et al. Exosomal Carboxypeptidase E (CPE) and CPE-shRNA-Loaded Exosomes Regulate Metastatic Phenotype of Tumor Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35328535
- Zhang C et al. Extracellular vesicles derived from mesenchymal stem cells alleviate neuroinflammation and mechanical allodynia in interstitial cystitis rats by inhibiting NLRP3 inflammasome activation. J Neuroinflammation 19:80 (2022). PubMed: 35387668
- Dong Q et al. Circular ribonucleic acid nucleoporin 98 knockdown alleviates high glucose-induced proliferation, fibrosis, inflammation and oxidative stress in human glomerular mesangial cells by regulating the microribonucleic acid-151-3p-high mobility group AT-hook 2 axis. J Diabetes Investig 13:1303-1315 (2022). PubMed: 35482475