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THP1 (human acute monocytic leukaemia cells).
Clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation
Our Abpromise guarantee covers the use of ab74080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"