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Overview

  • Product nameAnti-CD65s antibody [VIM-2] (FITC)See all CD65s primary antibodies ...
  • Description
    Mouse monoclonal [VIM-2] to CD65s (FITC)
  • ConjugationFITC
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    THP1 (human acute monocytic leukaemia cells).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityAmmonium Sulphate Precipitation
  • Primary antibody notes Clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation
  • Clonality Monoclonal
  • Clone numberVIM-2
  • MyelomaNS1
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab74080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceCD65s is aberrantly expressed on some acute myeloid leukaemias (AML). During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. CD65s is the sialylated form of human CD65 and is a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.
    • Alternative names
        CDw65 antibody

    Anti-CD65s antibody [VIM-2] (FITC) images

    • Flow Cytometry - CD65s antibody [VIM-2] (FITC) (ab74080)
      Flow Cytometry - CD65s antibody [VIM-2] (FITC) (ab74080)
      Staining of human peripheral blood granulocytes with ab74080 at 1/10 dilution.

    References for Anti-CD65s antibody [VIM-2] (FITC) (ab74080)

    This product has been referenced in:
    • Kniep B  et al. The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem 119:456-62 (1996). Read more (PubMed: 8830039) »

    See 1 Publication for this product

    Publishing research using ab74080 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"