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Read our guarantee »Anti-CD66a + CD66c + CD66d + CD66e antibody [CLB-gran/10, IH4Fc] (FITC)
See all CD66a + CD66c + CD66d + CD66e products (5) ...
Mouse monoclonal [CLB-gran/10, IH4Fc] to CD66a + CD66c + CD66d + CD66e (FITC)
FITC
ab51569 was conjugated with fluorescein isothiocyanate isomer 1 (FITC). The molecular F/P ratio is between 5 and 10.
ab51569 is directed against the CD66acde antigen (molecular mass 180- 200 kDa; phosphoprotein, 90 kDa; PI-linked protein), which is expressed on mature human granulocytes. After granulocyte activation the expression is strongly increased. The antibody reacts with 100% of mature human peripheral granulocytes. It reacts weakly with malignant cells of patients with B cell derived Chronic Lymphoid Leukaemia (CLL). ab51569 does not react with normal human peripheral B cells, T cells, monocytes and platelets.
Flow Cytmore details
Reacts with
Human
Human granulocytes
Liquid
Store at +4°C in the dark. Do not freeze.
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 20mM Tris, 150mM Sodium chloride, pH 8.0
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Protein G purified
ab51569 were purified from ascites or tissue culture medium using column chromatography (ion exchange and/or affinity chromatography).
This antibody would be useful for monitoring of mature granulocytes in peripheral blood.
Monoclonal
CLB-gran/10, IH4Fc
Sp2/0
IgG1
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Flow Cytometry - CD66a + CD66c + CD66d + CD66e antibody [CLB-gran/10, IH4Fc] (FITC) (ab51569)
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Our Abpromise guarantee covers the use of ab51569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 10µl for 40µl of 107 cells/ml suspension or 20µl per 100µl whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
CD66a, CD66c, CD66d and CD66e are four members of the carcinoembryonic antigen family and are expressed on human neutrophils. In neutrophils these proteins are activation antigens in that their surface expression is increased following stimulation.
Cell membrane; lipid anchor; GPI anchor
Flow Cytometry - CD66a + CD66c + CD66d + CD66e antibody [CLB-gran/10, IH4Fc] (FITC) (ab51569)
![Flow Cytometry - CD66a + CD66c + CD66d + CD66e antibody [CLB-gran/10, IH4Fc] (FITC) (ab51569)](/ps/datasheet/Images/51/ab51569/ab51569_FC.jpg)
Displays representative data performed on gated lymphocytes. Laser excitation is at 488 nm.
ab51569 has not yet been referenced specifically in any publications.
Publishing research using ab51569? Please let us know so that we can cite the reference in this datasheet
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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