CD68 antibody [ED1] (ab31630)

Product Name 

CD68 antibody [ED1]

Product type 

Primary antibodies

Description 

Mouse monoclonal [ED1] to CD68

Immunogen 

Tissue / cell preparation: Rat spleen cells

Reacts with 
(species key)

Rat

Specificity 

Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr, IHC-P, IP, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50 - 1/100. Use 10ul of the suggested working dilution to label 10 ⁶ cells in 100ul. See Damoiseaux et al.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/100. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
ICC: Use at an assay dependent dilution. See Damoiseaux et al.
IP: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. See Damoiseaux et al.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Type I membrane protein. Endosomal or lysosomal (Long isoform) and to a lesser extent on the cell surface (Short isoform).

Research areas 

Immunology >> Cell Type Markers >> CD >> Myeloid Cells

Relevance 

The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

ED1

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This product should be stored undiluted.

References for CD68 antibody [ED1] (ab31630)

  This product has been used in: (two most recent references)

Suuronen EJ et al. An acellular matrix-bound ligand enhances the mobilization, recruitment and therapeutic effects of circulating progenitor cells in a hindlimb ischemia model. FASEB J 23:1447-58 (2009). Rat. PubMed: 19136616

Salber D et al. Comparison of 99mTc- and 18F-ubiquicidin autoradiography to anti-Staphylococcus aureus immunofluorescence in rat muscle abscesses. J Nucl Med 49:995-9 (2008). Rat. PubMed: 18483088

See all 7 publication references for this product.


If you publish research using ab31630 please let us know so that we can cite the reference on this datasheet.

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