Anti-CD68 antibody [ED1] (ab31630)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer pH6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.

Image courtesy of an Abreview submitted by Dr. Carl Hobbs, King`s College London, United Kingdom

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  Immunohistochemistry (Frozen sections) - CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.

Image courtesy of an anonymous Abreview.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in mouse liver tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/200 in PBS and incubated with the sample for 1 hour 30 minutes. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody, diluted 1/250.

This image is courtesy of an Anonymous Abreview

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