You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-CD68 antibody [ED1] (ab31630)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-CD68 antibody [ED1]
See all CD68 products (17) ...

Description

Mouse monoclonal [ED1] to CD68

Specificity

Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.

Tested applications

Flow Cyt, IHC-Fr, IP, WB, RIA, ICC, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat

Immunogen

Tissue / cell preparation: Rat spleen cells

General notes

This product should be stored undiluted.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

ED1

Myeloma

Sp2/0-Ag14

Isotype

IgG1

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [ED1] (ab31630)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [ED1] (ab31630) image (enlarge)

  • Immunohistochemistry (Frozen sections) - CD68 antibody [ED1] (ab31630)Immunohistochemistry (Frozen sections) - CD68 antibody [ED1] (ab31630) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [ED1] (ab31630)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [ED1] (ab31630) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Tissue specificity

Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

Sequence similarities

Belongs to the LAMP family.

Post-translational
modifications

N- and O-glycosylated.

Cellular localization

Cell membrane and Endosome membrane. Lysosome membrane.

Target information above from: UniProt accessionP34810 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CD 68 antibody
  • CD68 antibody
  • CD68 antigen antibody
  • CD68 molecule antibody
  • CD68_HUMAN antibody
  • DKFZp686M18236 antibody
  • GP110 antibody
  • Macrophage antigen CD68 (microsialin) antibody
  • macrosialin antibody
  • SCARD1 antibody
  • Scavenger receptor class D member 1 antibody
see all

Anti-CD68 antibody [ED1] images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [ED1] (ab31630)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer pH6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.

Image courtesy of an Abreview submitted by Dr. Carl Hobbs, King`s College London, United Kingdom

See Abreview

  Immunohistochemistry (Frozen sections) - CD68 antibody [ED1] (ab31630)

Immunohistochemistry (Frozen sections) - CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.

Image courtesy of an anonymous Abreview.

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [ED1] (ab31630)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [ED1] (ab31630)

ab31630 staining CD68 in mouse liver tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% serum for 30 minutes at 25°C. The primary antibody was diluted 1/200 in PBS and incubated with the sample for 1 hour 30 minutes. A Biotin-conjugated Rabbit anti-Mouse polyclonal was used as the secondary antibody, diluted 1/250.

This image is courtesy of an Anonymous Abreview

See Abreview

References for Anti-CD68 antibody [ED1] (ab31630)

This product has been referenced in:

  • Salber Det al. Comparison of 99mTc- and 18F-ubiquicidin autoradiography to anti-Staphylococcus aureus immunofluorescence in rat muscle abscesses. J Nucl Med 49:995-9 (2008). Rat.Read more (PubMed: 18483088) »
  • Salber Det al. Differential uptake of O-(2-18F-fluoroethyl)-L-tyrosine, L-3H-methionine, and 3H-deoxyglucose in brain abscesses. J Nucl Med 48:2056-62 (2007). Rat.Read more (PubMed: 18006612) »

See all 11 publications for this product

Publishing research using ab31630? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"