Products:Immunology >> Cell Type Markers >> CD >> Myeloid Cells
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab53444? Please let us know so that we can cite the reference in this datasheet
ab53444 has been referenced in 9 publications.
Publishing research using ab53444? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Staining of permeabilised mouse peritoneal macrophages with Rat anti mouse CD68 (ab53444).
ab53444 staining mouse heart tissue by Immunohistochemistry (IHC-frozen sections). Tissue underwent fixation in paraformaldehyde, no permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody used undiluted and incubated with sample for 16 hours at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/100 was used as the secondary.
This image is courtesy of an Abreview submitted by Miss Silke Vorwald
ab53444 at 1/100 dilution staining CD68 in mouse spleen tissue by immunohistochemistry (frozen sections). Sections were acetone fixed prior to blocking in 8% milk for 40 minutes at 36ºC and then incubated with ab53444 for 20 hours at 4ºC. A biotin conjugated goat polyclonal to rat Ig, diluted 1/400, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
ab53444 staining CD68 in mouse spleen tissue by Immunohistochemistry (Frozen sections). Antibody was detected with HRP-conjugated Goat anti-Rat IgG, showing staining in the red pulp.
ab53444 staining CD68 in murine heart tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in acetone, permeabilized using 0.3% Triton, blocked with 10% serum for 30 minutes at 20°C, then incubated with ab53444 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated goat anti-rat polyclonal, used at a 1/1000 dilution.
This image is courtesy of an anonymous Abreview.
8
Call 01223 696 000 or contact us