Anti-CD68 antibody [FA-11] (ab53444)

  Flow Cytometry - CD68 antibody [FA-11] (ab53444)

Staining of permeabilised mouse peritoneal macrophages with Rat anti mouse CD68 (ab53444).

  Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)

ab53444 staining mouse heart tissue by Immunohistochemistry (IHC-frozen sections). Tissue underwent fixation in paraformaldehyde, no permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody used undiluted and incubated with sample for 16 hours at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/100 was used as the secondary.  

This image is courtesy of an Abreview submitted by Miss Silke Vorwald

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  Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)

ab53444 at 1/100 dilution staining CD68 in mouse spleen tissue by immunohistochemistry (frozen sections). Sections were acetone fixed prior to blocking in 8% milk for 40 minutes at 36°C and then incubated with ab53444 for 20 hours at 4°C. A biotin conjugated goat polyclonal to rat Ig, diluted 1/400, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

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  Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] (ab53444)

ab53444 staining CD68 in mouse spleen tissue by Immunohistochemistry (Frozen sections). Antibody was detected with HRP-conjugated Goat anti-Rat IgG, showing staining in the red pulp.

  Immunohistochemistry (Frozen sections) - Anti-CD68 antibody [FA-11] (ab53444)

ab53444 staining CD68 in murine heart tissue by Immunohistochemistry (Frozen sections).

Tissue was fixed in acetone, permeabilized using 0.3% Triton, blocked with 10% serum for 30 minutes at 20°C, then incubated with ab53444 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated goat anti-rat polyclonal, used at a 1/1000 dilution.

This image is courtesy of an anonymous Abreview.

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