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Flow Cytometry - CD68 antibody [FA-11] (ab53444)
![Flow Cytometry - CD68 antibody [FA-11] (ab53444)](/ps/datasheet/images/ab53444.1.jpg)
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Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)
![Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)](/ps/datasheet/images/ab53444_review_12193.jpg)
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Product Name
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CD68 antibody [FA-11]
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See all CD68 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [FA-11] to CD68
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Immunogen
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Tissue / cell preparation (Mouse)
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Reacts with
(species key)
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Ms
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Specificity
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ab53444 detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
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Tested applications
(see key)
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Flow Cyt, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution (PMID 18971331).
Flow Cyt: 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse peritoneal macrophages
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Cellular localization
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Type I membrane protein. Endosomal or lysosomal (Long isoform) and to a lesser extent on the cell surface (Short isoform).
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Research areas
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Immunology >> Cell Type Markers >> CD >> Myeloid Cells
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Relevance
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The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 12514 (Mouse)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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FA-11
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Isotype
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IgG2a
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD68 antibody [FA-11] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD68 antibody [FA-11] images, references, products related to ab53444 and other tools.
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CD68 antibody [FA-11] images:
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 Flow Cytometry - CD68 antibody [FA-11] (ab53444)
Staining of permeabilised mouse peritoneal macrophages with Rat anti mouse CD68 (ab53444).
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Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)
ab53444 staining mouse heart tissue by Immunohistochemistry (IHC-frozen sections). Tissue underwent fixation in paraformaldehyde, no permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody used undiluted and incubated with sample for 16 hours at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/100 was used as the secondary. This image is courtesy of an Abreview submitted by Miss Silke Vorwald
See Abreview
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References for CD68 antibody [FA-11] (ab53444)
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This product has been used in: (two most recent references)
Hamzah J et al. Targeted liposomal delivery of TLR9 ligands activates spontaneous antitumor immunity in an autochthonous cancer model. J Immunol 183:1091-8 (2009). IHC-Fr; Mouse. PubMed: 19561111
Wu J et al. HCO3-/Cl- anion exchanger SLC4A2 is required for proper osteoclast differentiation and function. Proc Natl Acad Sci U S A 105:16934-9 (2008). IHC-P; Mouse. PubMed: 18971331
See all 3 publication references for this product.
If you publish research using ab53444 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD68
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CD68 antibody [FA-11] - more information
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Customer reviews (feedback) regarding CD68 antibody [FA-11] |
Customer FAQs regarding CD68 antibody [FA-11] |
Protocols for CD68 antibody [FA-11] |
Price and availability of products related to CD68 antibody [FA-11] |
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CD68 antibody [FA-11] - related products:
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CD68 antibody [FA-11] - other tools:
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Contact Abcam with a Technical Enquiry about CD68 antibody [FA-11] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Flow Cytometry - CD68 antibody [FA-11] (ab53444)](/index.html?datasheet=53444#ab53444.1.jpg)
![Immunohistochemistry (Frozen sections) - CD68 antibody [FA-11] (ab53444)](/index.html?datasheet=53444#ab53444_review_12193.jpg)
