Anti-CD68 antibody [KP1] (ab955)

  Immunohistochemistry - CD68 antibody [KP1] (ab955)

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  Immunohistochemistry (Frozen sections) - CD68 antibody [KP1] (ab955)

ab955 at a 1/2000 dilution staining CD68 from mouse lung tissue sections (new born pups) by immunohistochemistry (frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa-Fluor® 488 Goat anti-mouse IgG. The negative control was secondary antibody alone.

This image is courtesy of an Abreview submitted by an anonymous researcher on 3 February 2006

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [KP1] (ab955)

ab955 staining CD68 in Human ulcerated Oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

This image is courtesy of an anonymous Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD68 antibody [KP1] (ab955)

ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

Image courtesy of an anonymous Abreview.

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