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Product Name
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CD68 antibody [PG-M1], prediluted
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See all CD68 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [PG-M1] to CD68, prediluted
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Immunogen
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BALB/C mice were immunized with Gaucher’s cells.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule.
It stains macrophages in a wide variety of tissues.
Myeloid precursor cells and peripheral granulocytes are negative.
This antibody does not react with granulocytes and their precursor cells.
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Tested applications
(see key)
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
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Cellular localization
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Type I membrane protein. Endosomal or lysosomal (Long isoform) and to a lesser extent on the cell surface (Short isoform).
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Research areas
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Immunology >> Cell Type Markers >> CD >> Myeloid Cells
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Relevance
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The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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PG-M1
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Isotype
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IgG1
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Light chain type
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kappa
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: Inert stabilizer Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD68 antibody [PG-M1], prediluted is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CD68
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CD68 antibody [PG-M1], prediluted - more information
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