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Products:Immunology >> Cell Type Markers >> CD >> Myeloid Cells
Overview
Product name
Anti-CD68 antibody [PG-M1], prediluted
See all CD68 products (17) ...
Description
Mouse monoclonal [PG-M1] to CD68, prediluted
Specificity
This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
BALB/C mice were immunized with Gaucher’s cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Inert stabilizer
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
PG-M1
Myeloma
unknown
Isotype
IgG1
Light chain type
kappa
Research areas
Immunology >> Cell Type Markers >> CD >> Myeloid Cells
Applications
Show applications key
Our Abpromise guarantee covers the use of ab844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
Application notes
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
Target
Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similarities
Belongs to the LAMP family.
Post-translational
modifications
N- and O-glycosylated.
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane.
Target information above from: UniProt accessionP34810
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
References for Anti-CD68 antibody [PG-M1], prediluted (ab844)
ab844 has not yet been referenced specifically in any publications.
Publishing research using ab844? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"