FITC Anti-CD68 antibody [Y1/82A] (ab134351)
Key features and details
- FITC Mouse monoclonal [Y1/82A] to CD68
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD68 antibody [Y1/82A]
See all CD68 primary antibodies -
Description
FITC Mouse monoclonal [Y1/82A] to CD68 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Normal lymphocytes stimulated with phytohaemagluttin/Freund's adjuvant
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Positive control
- Flow Cyt (Intra): Human peripheral blood monocytes
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General notes
The reagent is free of unconjugated FITC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 98% PBS, 0.2% BSA -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Purified by affinity chromatography. Purity of this monoclonal antibodies is 95%. -
Clonality
Monoclonal -
Clone number
Y1/82A -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
Use 5-20µl for 106 cells.
in 100 µl staining volume. Or Use 5 - 20 µl for 100 µl of whole blood.
ab18427 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt (Intra)
Use 5-20µl for 106 cells. in 100 µl staining volume. Or Use 5 - 20 µl for 100 µl of whole blood.
ab18427 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
Datasheets and documents
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Datasheet download
References (4)
ab134351 has been referenced in 4 publications.
- Gu S et al. AKT3 deficiency in M2 macrophages impairs cutaneous wound healing by disrupting tissue remodeling. Aging (Albany NY) 12:6928-6946 (2020). PubMed: 32291381
- Xiao P et al. Phosphatase Shp2 exacerbates intestinal inflammation by disrupting macrophage responsiveness to interleukin-10. J Exp Med 216:337-349 (2019). PubMed: 30610104
- Feng Z et al. Tumor-associated macrophage-derived exosomal microRNA-155-5p stimulates intracranial aneurysm formation and macrophage infiltration. Clin Sci (Lond) 133:2265-2282 (2019). PubMed: 31657855
- Yin J et al. Role of P2X7R in the development and progression of pulmonary hypertension. Respir Res 18:127 (2017). IHC-P ; Rat . PubMed: 28646872