Anti-CD69 antibody [FN50] (ab51862)
Key features and details
- Mouse monoclonal [FN50] to CD69
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD69 antibody [FN50]
See all CD69 primary antibodies -
Description
Mouse monoclonal [FN50] to CD69 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD69. Stimulated human B-lymphocytes (a cell suspension).
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General notes
Original vial: 1 year at 4° - 8°C Stock solution or aliquots: 1 year at -20°C. Avoid repeated thawing and freezing.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute by adding 0.5ml distilled water. -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: BSA, PBS, Water -
Concentration information loading...
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Purification notes
Purified from tissue culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
FN50 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 969 Human
- Omim: 107273 Human
- SwissProt: Q07108 Human
- Unigene: 208854 Human
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Alternative names
- Activation inducer molecule (AIM/CD69) antibody
- Activation inducer molecule antibody
- AIM antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab51862 has been referenced in 3 publications.
- Engmann J et al. Increased Activity and Apoptosis of Eosinophils in Blister Fluids, Skin and Peripheral Blood of Patients with Bullous Pemphigoid. Acta Derm Venereol 97:464-471 (2017). PubMed: 27868136
- Henderson NC et al. CD98hc (SLC3A2) interaction with beta 1 integrins is required for transformation. J Biol Chem 279:54731-41 (2004). PubMed: 15485886
- Vance BA et al. Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-cys) glycosylation motifs. J Biol Chem 272:23117-22 (1997). PubMed: 9287313