Anti-CD69 antibody [H1.2F3] (PE/Cy5®) (ab25491)
Overview
- Product nameAnti-CD69 antibody [H1.2F3] (PE/Cy5®)See all CD69 primary antibodies ...
- DescriptionArmenian Hamster monoclonal [H1.2F3] to CD69 (PE/Cy5®)
- ConjugationPE/Cy5®
- Tested applicationsFlow Cyt, IHC-Fr, IP, Cellular activation more details
- Species reactivityReacts with: Mouse
- Immunogen
Tissue / cell preparation: Y245 mouse dendritic epidermal T cell line.
- General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 16% Sucrose, 0.20% Gelatin, PBS and stabilising agent. -
Concentration information loading... - PurityIgG fraction
- Primary antibody notes This antibody augments PMA induced T cell proliferation, and induces redirected lysis of Fc receptor bearing target cells by NK cells.
- Clonality Monoclonal
- Clone numberH1.2F3
- IsotypeIgG
- Light chain typelambda
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab25491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | |
| IHC-Fr | |
| IP | |
| Cellular activation |
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
In vitro T cell and NK cell activation: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionInvolved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
- Tissue specificityExpressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
- Sequence similaritiesContains 1 C-type lectin domain.
- Developmental stageEarliest inducible cell surface glycoprotein acquired during lymphoid activation.
- Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. - Cellular localizationMembrane.
-
Database links
- Entrez Gene: 12515 Mouse
- SwissProt: P37217 Mouse
- Unigene: 74745 Mouse
Target information above from: UniProt accession
Q07108
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Activation inducer molecule (AIM/CD69) antibodyActivation inducer molecule antibodyAIM antibody
- BL-AC/P26 antibodyBLAC/P26 antibodyC-type lectin domain family 2 member C antibodyCD69 antibodyCD69 antigen (p60, early T-cell activation antigen) antibodyCD69 antigen antibodyCD69 molecule antibodyCD69_HUMAN antibodyCLEC2C antibodyEA1 antibodyEarly activation antigen CD69 antibodyEarly lymphocyte activation antigen antibodyEarly T cell activation antigen p60 antibodyEarly T-cell activation antigen p60 antibodyGP32/28 antibodyLeu23 antibodyLeukocyte surface antigen Leu-23 antibodyMLR-3 antibodyMLR3 antibody
see all
References for Anti-CD69 antibody [H1.2F3] (PE/Cy5®) (ab25491)
This product has been referenced in:
- Sobel ES et al. Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes. J Immunol 150:673-82 (1993). Read more (PubMed: 8380429) »
- Karlhofer FM & Yokoyama WM Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol 146:3662-73 (1991). Read more (PubMed: 2026886) »
