Recombinant Anti-CD7 antibody [EPR4242] - BSA and Azide free (ab230834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4242] to CD7 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD7 antibody [EPR4242] - BSA and Azide free
See all CD7 primary antibodies -
Description
Rabbit monoclonal [EPR4242] to CD7 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.
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Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): Jurkat cells; IP: Human spleen lysate; ICC/IF: Jurkat cells; IHC-P: Rat and mouse spleen tissues. Human colon carcinoma and tonsil tissues; WB: Human spleen lysate,
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General notes
ab230834 is the carrier-free version of ab109296.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4242 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD7 antibody [EPR4242] (ab109296)
- Alexa Fluor® 488 Anti-CD7 antibody [EPR4242] (ab199022)
- Alexa Fluor® 647 Anti-CD7 antibody [EPR4242] (ab199023)
- Alexa Fluor® 594 Anti-CD7 antibody [EPR4242] (ab277233)
- Alexa Fluor® 555 Anti-CD7 antibody [EPR4242] (ab279334)
- PE Anti-CD7 antibody [EPR4242] (ab303140)
- APC Anti-CD7 antibody [EPR4242] (ab303141)
- HRP Anti-CD7 antibody [EPR4242] (ab303142)
- Alexa Fluor® 568 Anti-CD7 antibody [EPR4242] (ab312617)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).
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IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa). |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Not yet known. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 924 Human
- Entrez Gene: 12516 Mouse
- Entrez Gene: 303747 Rat
- Omim: 186820 Human
- SwissProt: P09564 Human
- SwissProt: P50283 Mouse
- Unigene: 36972 Human
- Unigene: 4100 Mouse
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Alternative names
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
Images
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Anti-CD7 antibody [EPR4242] (ab109296) at 1/10000 dilution + Human Spleen Lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?This data was developed using ab109296, the same antibody clone in a different buffer formulation.
The molecular mass observed is consistent with what has been described in the literatures (PMID: 24157461, 2479685).
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This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purifiedab109296 at 1/100 dilution (1�g) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This data was developed using ab109296, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This data was developed using ab109296, the same antibody clone in a different buffer formulation.
CD7 was immunoprecipitated from 0.35 mg Human spleen lysate 10 ug with purified ab109296 at 1/50. Western blot was performed on the immunoprecipitate using ab109296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human spleen lysate 10 ug
Lane 2: ab109296 IP in Human spleen lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109296 in Human spleen lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109296).
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Tissue Microarrays stained for " Anti-CD7 antibody [EPR4242]” using " ab109296" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab109296 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab230834 has not yet been referenced specifically in any publications.