Anti-CD73 antibody - C-terminal (ab71822)

Overview

• Product nameAnti-CD73 antibody - C-terminalSee all CD73 primary antibodies ...
• Description
  Rabbit polyclonal to CD73 - C-terminal
• Tested applicationsELISA, IHC-P, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Positive controlHuman breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab71822 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Leukocyte recruitment
  • Other

  • Cardiovascular
  • Hypoxia
  • Associated Proteins

Applications

Target

• FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
• Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
• Sequence similaritiesBelongs to the 5'-nucleotidase family.
• Cellular localizationCell membrane.

Target information above from: UniProt accession P21589 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4907 Human
  • Entrez Gene: 23959 Mouse
  • Omim: 129190 Human
  • SwissProt: P21589 Human
  • SwissProt: Q61503 Mouse
  • Unigene: 153952 Human
  • Unigene: 244235 Mouse

• Alternative names
  5' NT antibody5' nucleotidase (CD73) antibody5' nucleotidase precursor antibody

  5' nucleotidase, ecto antibody5' nucleotidase, ecto (CD73) antibody5'-NT antibody5'-nucleotidase antibody5NTD_HUMAN antibodyCD73 antibodyCD73 antigen antibodyE5NT antibodyEcto 5' nucleotidase antibodyEcto-5'-nucleotidase antibodyeN antibodyeNT antibodyNT antibodyNT5 antibodyNT5E antibodyNTE antibodyPurine 5 Prime Nucleotidase antibody

  see all

Anti-CD73 antibody - C-terminal images

• 

  Western blot - CD73 antibody - C-terminal (ab71822)
  Anti-CD73 antibody - C-terminal (ab71822) at 1/100 dilution + Mouse brain tissure lysate at 35 µg
  
  Predicted band size : 63 kDa
  Observed band size : 63 kDa
  Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD73 antibody - C-terminal (ab71822)
  ab71822 at 1/50 dilution, staining CD73 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.