Overview

  • Product nameAnti-CD73 antibody - C-terminalSee all CD73 primary antibodies ...
  • Description
    Rabbit polyclonal to CD73 - C-terminal
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Positive control
    • Human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71822 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab71822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similaritiesBelongs to the 5'-nucleotidase family.
  • Cellular localizationCell membrane.
  • Target information above from: UniProt accession P21589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody - C-terminal images

  • Anti-CD73 antibody - C-terminal (ab71822) at 1/100 dilution + Mouse brain tissure lysate at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab71822 at 1/50 dilution, staining CD73 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-CD73 antibody - C-terminal (ab71822)

This product has been referenced in:
  • Kurth TB  et al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum 63:1289-300 (2011). Read more (PubMed: 21538315) »

See 1 Publication for this product

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