The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
Use at an assay dependent concentration. PubMed: 25375632
FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
Sequence similaritiesBelongs to the 5'-nucleotidase family.
ab71822 at 1/50 dilution, staining CD73 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
References for Anti-CD73 antibody - C-terminal (ab71822)
This product has been referenced in:
Clauser L et al. Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery. PLoS One9:e110796 (2014).
Read more (PubMed: 25375632) »
Kurth TB et al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum63:1289-300 (2011).
Read more (PubMed: 21538315) »