Anti-CD74 antibody [CLIP/813] (ab215898)
Key features and details
- Mouse monoclonal [CLIP/813] to CD74
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD74 antibody [CLIP/813]
See all CD74 primary antibodies -
Description
Mouse monoclonal [CLIP/813] to CD74 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD74 aa 1-300.
Database link: P04233 -
Positive control
- IHC-P: Human tonsil tissue. Flow cyt: Raji cells ICC/IF: Raji cells. WB: Raji cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CLIP/813 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 34 kDa.
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Flow Cyt |
Use 1-2µg for 106 cells.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 34 kDa. |
Flow Cyt
Use 1-2µg for 106 cells. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. -
Sequence similarities
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 972 Human
- Omim: 142790 Human
- SwissProt: P04233 Human
- Unigene: 436568 Human
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Alternative names
- CD 74 antibody
- CD74 antibody
- CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
see all
Images
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Anti-CD74 antibody [CLIP/813] (ab215898) at 4 µg/ml + Raji cell lysate
Predicted band size: 34 kDa -
Immunofluorescent staining of Raji cells using ab215898 followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD74 with ab215898 at 2 µg/mL.
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Flow Cytometric Analysis of Human Raji cells using ab215898 followed by goat anti-Mouse IgG-CF488 (Blue), and Isotype Control (Red).
Datasheets and documents
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Datasheet download
References (0)
ab215898 has not yet been referenced specifically in any publications.