Anti-CD74 antibody [LN2] (ab9514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD74 antibody [LN2] (ab9514)

ab9514 staining human tonsil by IHC-P.

Western blot - CD74 antibody [LN2] (ab9514)

Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Tonsil (Human) Whole Cell Lysate - adult normal tissue (ab29615) at 10 µg

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size : 34 kDa
Observed band size : 34 kDa
Additional bands at : 37 kDa (possible post-translational modification).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD74 antibody [LN2] (ab9514)

ab9514 staining CD74 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/75 dilution for 30 minutes at 20°C. A HRP-conjugated rat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

Image courtesy of an anonymous Abreview.

Flow Cytometry-CD74 antibody [LN2](ab9514)

Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.