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The details of the immunogen for this antibody are not available.
ab21419 detects Ia antigens from rat strains Wistar, PVG, Sprague Dawley, Lewis and AO. Other strains have not been tested. The antibody labels B lymphocytes, various epithelial cells, dendritic cells and macrophages. T lymphocytes are not labelled with this antibody. ab21419 detects Ia specificity 17 from the mouse H-2 haplotypes f,k,r,s and u but not with b,d,p,q or v haplotypes. ab21419 can be used for the purification of Ia antigen from rat spleen.
Our Abpromise guarantee covers the use of ab21419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/5 - 1/25.
Periodate lysine paraformaldehyde fixed.
|Flow Cyt||1/5 - 1/25. Ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"